LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
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B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.
APRIL 2007
VS 423 – RECOMBINANT DNA TECHNOLOGY
Date & Time: 28/04/2007 / 1:00 – 4:00 Dept. No. Max. : 100 Marks
Part A (10 x 2 = 20)
Write short notes on the following:
- DNA methylase
- Palindromic sequences
- Reverse transcriptase
- Selectable markers
- DNA primer
- Jumping genes
- Ti plasmid
- Hb vaccine
- “Super bug”
- Intellectual Property Rights
Part B (5 x 8 = 40)
Answer any FIVE of the following, not exceeding 350 words each. Draw necessary diagrams.
- Comment on restriction and modification systems of E. coli.
- Give the protocol for restriction mapping of DNA.
- Bring out the strategies for the construction of genomic DNA library.
- Write notes on various expressions of cloned genes.
- Explain restriction fragment length polymorphism and its applications.
- Describe Maxam and Gilbert DNA sequencing process.
- Enumerate and explain the International Safety Guidelines.
Part – C (2 x 20 = 40)
Answer the following in not more than 1500 words. Draw necessary diagrams.
- (a) Describe the classification of restriction enzymes. Give suitable examples.
Or
(b) Explain the types of vectors used in recombinant DNA technology.
- (a) Write detailed notes on industrial applications of rDNA technology.
Or
(b) Bring out the social and moral implications of genetic engineering.
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